Crystallization　of　two　enantiomers　Δ-1　and　Λ-1　was　achieved　by　using　an　achiral　flexible　ligand　H4L　(2,2＇-[1,2-ethanediylbis[(hydroxyethylimino)methylene]]bis[6-methoxy-4-methyl-phenol]).　The　absolute　configurations　of　the　enantiomers　in　P3121　(Δ-1)　and　P3221　(Λ-1)　are　confirmed　by　the　X-ray　single　crystal　diffraction.　Solid-state　circular　dichroism　spectra　show　that　two　enantiomers　have　absorption　peaks　of　mirror　symmetry.　The　experimental　energy　barrier　of　88.9　K　derived　from　the　alternating-current　magnetic　measurements　is　consistent　with　the　energy　gap　(89.9　K)　between　two　lowest　Kramers　doublets　calculated　by　ab　initio　method.　©　2018　American　Chemical　Society.