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author：

Li, Z. (Li, Z..) [1] | Chen, H. (Chen, H..) [2] | Zhuo, Z. (Zhuo, Z..) [3] | Huang, D. (Huang, D..) [4] | Luo, F. (Luo, F..) [5] | Chen, L. (Chen, L..) [6] | Wang, J. (Wang, J..) [7] | Guo, L. (Guo, L..) [8] | Qiu, B. (Qiu, B..) [9] | Lin, Z. (Lin, Z..) [10]

Indexed by：

Scopus

Abstract：

The　development　of　simple　and　sensitive　method　for　hyaluronidase　(HAase)　detection　is　significant　for　the　clinical　diagnosis　and　treatment　of　cancer　as　HAase　is　a　potential　tumor　marker.　In　this　study,　a　simple　but　sensitive　electrochemiluminescence　(ECL)　biosensor　has　been　designed　for　HAase　detection　based　on　the　electrostatic　interaction　of　anionic　hyaluronic　acid　(HA)　and　cationic　luminous　reagent　Ru(bpy)3　2+.　Ru(bpy)3　2+　is　adsorbed　on　HA　through　electrostatic　interaction　to　form　HA-Ru(bpy)3　2+　complex.　The　Ru(bpy)3　2+　in　the　HA-Ru(bpy)3　2+　complex　cannot　be　filtered　out　because　of　its　large　molecular　weight.　In　the　presence　of　HAase,　the　HA　in　HA-Ru(bpy)3　2+　can　be　cleaved　into　fragments　by　enzymatic　hydrolysis.　As　a　result,　the　Ru(bpy)3　2+　attached　on　the　HA　fragments　can　be　separated　from　the　HA-Ru(bpy)3　2+　complex　by　centrifugation　easily.　The　resulting　ultrafiltrate　containing　Ru(bpy)3　2+　can　be　used　to　characterize　the　concentration　of　HAase　in　the　sample.　The　ECL　intensity　of　Ru(bpy)3　2+　has　a　linear　relationship　with　the　concentration　of　HAase　in　the　range　of　2.0–40　U/mL　with　a　detection　limit　of　0.33　U/mL.　The　proposed　ECL　system　has　been　applied　to　detect　HAase　in　urine　samples　with　satisfied　results　and　HAase　inhibitor　with　high　efficiency.　©　2018　Elsevier　B.V.

Keyword：

Electrochemiluminescence; Hyaluronic acid; Hyaluronidase; Ru(bpy)3 2+

Community：

  • [ 1 ] [Li, Z.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
  • [ 2 ] [Chen, H.]Department of Hepatobiliary Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian 350000, China
  • [ 3 ] [Zhuo, Z.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
  • [ 4 ] [Huang, D.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350116, China
  • [ 5 ] [Luo, F.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
  • [ 6 ] [Luo, F.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350116, China
  • [ 7 ] [Chen, L.]Department of Gastroenterology, Ruijin Hospital North, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China
  • [ 8 ] [Wang, J.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
  • [ 9 ] [Guo, L.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
  • [ 10 ] [Qiu, B.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
  • [ 11 ] [Lin, Z.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China

Reprint 's Address：

  • [Luo, F.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Biological Science and Engineering, Fuzhou UniversityChina

Email：


luofang0812@163.com
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Source ：

Sensors and Actuators, B: Chemical

ISSN： 0925-4005

Year： 2018

Volume： 275

Page： 409-414

6 . 3 9 3

JCR@2018

6 . 3 9 3

JCR@2018

JCR Journal Grade：1

CAS Journal Grade：1

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 18

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 1

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