A novel purification procedure for recombinant human serum albumin expressed in Pichia pastoris - Details

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Abstract：

Plasma-derived　human　serum　albumin　(pHSA)　has　important　applications　in　many　clinical　indications,　including　blood　loss,　serious　burn,　or　hemorrhagic　shock.　The　limited　supply　and　potential　infectious　pathogen　contamination　of　pHSA　have　stimulated　the　development　of　recombinant　human　serum　albumin　(rHSA).　However,　rHSA　often　entraps　endogenous　or　exogenous　impurities,　including　color　pigments　and　polysaccharides.　Therefore,　the　purification　of　rHSA　to　high　purity　remains　the　bottleneck　in　large-scale　production　of　rHSA.　Herein　we　report　a　novel　two-step　purification　protocol　for　rHSA　generated　from　Pichia　pastoris.　In　the　first　step,　rHSA　was　partially　unfolded　to　expose　impurities　entrapped　into　rHSA　and　was　then　removed.　In　the　second　step,　rHSA　was　crystallized　to　further　remove　impurities.　Through　this　procedure,　the　pigment　content　(A350/A280)　and　polysaccharides　content　of　rHSA　were　reduced　to　0.0141　and　0.253　μg/mg,　respectively,　which　were　comparable　to　pHSA.　In　addition,　the　secondary　structure　and　bioactivity　of　purified　rHSA　are　preserved.　The　purification　procedure　developed　in　this　study　is　a　simple,　short　and　low　cost　method　to　produce　active　rHSA,　or　rHSA-fusion　proteins,　to　high　purity,　and　also　suitable　for　the　purification　of　other　disulfide-rich　proteins.　©　2018　Elsevier　Inc.

Keyword：

Crystallization; Impurities; Purification; Recombinant human serum albumin

Community：

[ 1 ] [Li, S.]State Key Laboratory of Structure Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China
[ 2 ] [Li, S.]College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
[ 3 ] [Li, S.]College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
[ 4 ] [Li, L.]College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
[ 5 ] [Chen, Z.]State Key Laboratory of Structure Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China
[ 6 ] [Xue, G.]State Key Laboratory of Structure Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China
[ 7 ] [Xue, G.]College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
[ 8 ] [Jiang, L.]College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
[ 9 ] [Zheng, K.]College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China
[ 10 ] [Chen, J.]State Key Laboratory of Structure Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China
[ 11 ] [Li, R.]State Key Laboratory of Structure Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China
[ 12 ] [Yuan, C.]College of Bioscience and Biotechnology, Fuzhou University, Fuzhou, Fujian 350116, China
[ 13 ] [Huang, M.]State Key Laboratory of Structure Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China
[ 14 ] [Huang, M.]College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China

Reprint 's Address：

[Huang, M.]State Key Laboratory of Structure Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of SciencesChina

Email：

mhuang@fjirsm.ac.cn

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Source ：

Protein Expression and Purification

ISSN： 1046-5928

Year： 2018

Volume： 149

Page： 37-42

1 . 2 9 1

JCR@2018

1 . 4 0 0

JCR@2023

ESI HC Threshold：212

JCR Journal Grade：4

CAS Journal Grade：4

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 10

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 1

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