A　hydrophilic　ionic　liquids　based　polymer　monolith　microextraction　(PMME)　column　(poly(ionic　liquid-co-hydroxyethyl　methacrylate-co-ethylene　dimethacrylate);　poly(IL-co-HEMA-co-EDMA))　was　prepared　for　the　first　time　in　a　capillary　and　utilized　in　PMME　for　the　enrichment　of　glycopeptide　antibiotics　(GAs),　followed　by　the　online　coupling　analysis　of　capillary　liquid　chromatography　with　amperometric　detection　(cLC-AD).　The　prepared　monolith　exhibited　large　through　pores　and　good　storage　stability,　as　well　as　a　selective　extraction　machanism　for　GAs　that　was　attributed　to　the　hydrogen　bonding,　hydrophilic,　electrostatic　and　π-π　interactions　between　GAs　and　the　imidazolium　cations　or　hydroxyl　groups　on　the　surface　of　absorbent.　Several　experimental　parameters,　such　as　sample　flow　rate,　composition　of　eluent　and　solvent　desorption　conditions,　were　examined　to　improve　the　extraction　efficiency　of　PMME.　Under　the　optimal　conditions,　the　proposed　PMME-cLC-AD　method　provides　detection　limits　(S/N　=　3)　of　1.0–8.0　μg　L−1　for　three　GAs,　which　are　1000-fold　lower　than　those　obtained　by　cLC-AD,　with　a　wide　linear　range　of　10.0–12000.0　μg　L−1.　It　was　successfully　applied　for　the　analysis　of　GAs　residues　in　feed　samples　with　good　recoveries　(80.3–119.1%)　and　satisfied　intra-day/inter-day　precision　(<10%).　Compared　with　LC-3Q-MS　method,　the　proposed　online　approach　has　the　merits　of　simple,　low　cost,　smaller　matrix　interference　and　environmental　friendly,　which　is　demonstrated　to　be　a　feasible　tool　for　residue　analysis　of　peptide　antibiotics　in　food　safety　application.　©　2018　Elsevier　B.V.