A homogeneous electrochemical sensor for Hg2+ determination in environmental water based on the T-Hg2+-T structure and exonuclease III-assisted recycling amplification - Details

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Abstract：

A　simple,　fast,　sensitive,　and　homogeneous　electrochemical　sensor　based　on　the　T-Hg2+-T　structure　and　exonuclease　III-assisted　recycling　amplification　has　been　constructed　for　mercury　ion　(Hg2+)　detection.　The　cT　and　methylene　blue-labeled　DNA　probes　(MB-TDNA)　were　designed　to　contain　poly　T　sequences,　which　were　repulsed　from　the　negatively　charged　indium　tin　oxide　(ITO)　electrode　due　to　their　abundant　negative　charges.　Hg2+　could　trigger　the　formation　of　double-stranded　DNA　(dsDNA)　between　two　DNA　probes　owing　to　the　stable　T-Hg2+-T　structure.　Then,　Exo　III　specifically　recognizes　the　cleavage　of　the　double-stranded　structure　to　release　a　methylene　blue-labeled　mononucleotide　fragment　(MB-MF).　Moreover,　the　release　of　the　target　Hg2+　induces　new　hybridization　and　produces　a　large　number　of　MB-MFs;　MB-MFs　are　not　repulsed　by　the　negatively　charged　ITO　electrode　surface,　thus　producing　a　significant　current　signal.　Under　optimal　conditions,　the　differential　pulse　voltammetric　(DPV)　response　had　a　linear　relationship　with　the　logarithm　of　Hg2+　concentration　in　the　range　of　1.0　nM-0.5　μM,　and　the　proposed　method　displayed　great　applicability　for　detecting　Hg2+　in　tap-water　samples.　©　2018　The　Royal　Society　of　Chemistry.

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[ 1 ] [Fu, C.]College of Biological Sciences and Engineering, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
[ 2 ] [Fu, C.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
[ 3 ] [Yu, H.]Department of Prosthodontics, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, 350005, China
[ 4 ] [Su, L.]College of Biological Sciences and Engineering, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
[ 5 ] [Liu, C.]College of Biological Sciences and Engineering, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
[ 6 ] [Song, Y.]College of Biological Sciences and Engineering, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
[ 7 ] [Wang, S.]College of Biological Sciences and Engineering, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
[ 8 ] [Lin, Z.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
[ 9 ] [Chen, F.]College of Food Science and Nutritional Engineering, National Engineering Research Centre for Fruits and Vegetables Processing, Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, China Agricultural University, Beijing, 100083, China

Reprint 's Address：

[Lin, Z.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou UniversityChina

Email：

zylin@fzu.edu.cn

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Analyst

ISSN： 0003-2654

Year： 2018

Issue： 9

Volume： 143

Page： 2122-2127

4 . 0 1 9

JCR@2018

3 . 6 0 0

JCR@2023

ESI HC Threshold：209

JCR Journal Grade：1

CAS Journal Grade：2

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SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 4

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