DNA　methylation　is　closely　associated　with　aberrant　epigenetic　changes.　Previous　studies　have　identified　various　genes　associated　with　non-small　cell　lung　cancer　(NSCLC),　but　the　precise　combination　responsible　for　its　etiology　is　still　debated.　The　aim　of　the　present　study　was　to　select　a　new　set　of　NSCLC-related　genes　using　methylation-sensitive　high-resolution　melting.　The　promoter　methylation　status　of　six　selected　genes,　consisting　of　protocadherin　γ　subfamily　B,　6　(PCDHGB6),　homeobox　A9　(HOXA9),　O6　-methylguanine-DNA　methyltransferase　(MGMT),　microRNA　(miR)-126,　suppressor　of　cytokine　signaling　3　(SOCS3)　and　Ras　association　domain　family　member　5,　also　termed　NORE1A,　was　evaluated　in　54　NSCLC　patients.　From　these　samples,　genome-wide　DNA　was　extracted　and　bisulfite　conversion　was　performed　along　with　fluorogenic　quantitative　polymerase　chain　reaction　to　detect　methylation　values　of　the　six　selected　promoters.　The　present　results　revealed　frequent　methylation　on　PCDHGB6,　HOXA9　and　miR-126,　which　contrasted　with　infrequent　methylation　on　MGMT.　The　results　indicated　no　methylation　on　either　SOCS3　or　NORE1A.　The　sensitivity　and　specificity　of　the　methylation　assessment　were　85.2　and　81.5%,　respectively,　and　the　analysis　results　were　validated　by　pyrosequencing.　Furthermore,　minute　comparison　of　the　association　between　DNA　methylation　and　clinical　features　was　performed.　Overall,　these　results　may　provide　potential　information　for　the　development　of　better　clinical　diagnostics　and　more　targeted　and　effective　therapies　for　NSCLC.　©　2018,　Spandidos　Publications.　All　rights　reserved.