Nongenetic Approach for Imaging Protein Dimerization by Aptamer Recognition and Proximity-Induced DNA Assembly - Details

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Abstract：

Herein,　we　report　a　nongenetic　and　real-time　approach　for　imaging　protein　dimerization　on　living　cell　surfaces　by　aptamer　recognition　and　proximity-induced　DNA　assembly.　We　use　the　aptamer　specific　for　the　receptor　monomer　as　a　recognition　probe.　When　receptor　dimerization　occurs,　the　dimeric　receptors　bring　two　aptamer　probes　into　close　proximity,　thereby　triggering　dynamic　DNA　assembly.　The　proposed　approach　was　successfully　applied　to　visualize　dimerization　of　Met　receptor　and　transforming　growth　factor-β　type　II　receptor.　This　approach　allows　us　to　image　the　two　states　(monomer/dimer)　of　a　receptor　protein　on　living　cell　surfaces　in　real　time,　opening　a　universal　method　for　further　investigation　of　protein　dimerization　and　the　corresponding　activation　processes　in　signal　transduction.　©　2018　American　Chemical　Society.

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[ 1 ] [Liang, H.]MOE, Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 2 ] [Chen, S.]MOE, Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 3 ] [Li, P.]MOE, Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 4 ] [Wang, L.]MOE, Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 5 ] [Le, J.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350116, China
[ 6 ] [Li, J.]MOE, Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 7 ] [Li, J.]Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China
[ 8 ] [Yang, H.-H.]MOE, Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
[ 9 ] [Yang, H.-H.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350116, China
[ 10 ] [Tan, W.]Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China
[ 11 ] [Tan, W.]Department of Chemistry, Center for Research at the Bio/Nano Interface, UF Health Cancer Center, University of Florida, Gainesville, FL 32611-7200, United States

Reprint 's Address：

[Li, J.]MOE, Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou UniversityChina

Email：

lijuan@fzu.edu.cn

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Source ：

Journal of the American Chemical Society

ISSN： 0002-7863

Year： 2018

Issue： 12

Volume： 140

Page： 4186-4190

1 4 . 6 9 5

JCR@2018

1 4 . 5 0 0

JCR@2023

ESI HC Threshold：209

JCR Journal Grade：1

CAS Journal Grade：1

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ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 1

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