In　this　work,　metal-organic　framework　particles　incorporated　fibers　(UiO-66/PAN　nanofibers)　were　used　as　adsorbent　in　pipette　tip　solid　phase　extraction　(PT-SPE)　for　the　first　time.　The　UiO-66/PAN　nanofibers　were　fabricated　by　a　facile　electrospinning　method　and　characterized　by　Fourier　transform　infrared　spectroscopy,　scanning　electron　microscopy,　X-ray　diffraction　and　nitrogen　adsorption-desorption　experiments.　The　UiO-66/PAN　nanofibers　were　applied　to　assemble　a　novel　PT-SPE　cartridge　for　determination　of　four　phytohormones　followed　by　high　performance　liquid　chromatography　(HPLC).　Several　experimental　parameters　such　as　kinds　of　UiO-66/PAN　nanofibers,　the　amount　of　UiO-66/PAN　nanofibers,　the　effect　of　solution　pH,　ionic　strength　and　desorption　conditions　were　intensively　investigated.　Under　the　optimal　conditions,　the　linear　ranges　of　the　phytohormones　were　in　the　range　of　0.06–60　ng/mL　with　correlation　coefficients　above　0.992.　The　limits　of　detection　were　between　0.01　ng/mL　to　0.02　ng/mL.　The　interday　and　intraday　precision　(RSD)　for　three　replicate　extractions　of　the　four　phytohormones　(15　ng/mL　for　each)　was　in　the　range　of　1.5–5.6%.　The　established　method　was　successfully　applied　for　the　determination　of　phytohormones　in　watermelon　and　mung　bean　sprouts　samples.　The　results　showed　that　the　electrostatic　interaction　between　the　positively　charged　UiO-66　and　anionic　forms　of　phytohormones　played　an　important　role　in　the　extraction　of　the　phytohormones.　©　2018　Elsevier　B.V.