A　rapid　and　sensitive　method　was　established　for　the　analysis　of　peptide　antibiotics　(bacitracin,　polymyxin　B　and　colistin)　in　animal　food　by　capillary　electrochromatography　(CEC)　coupled　with　laser　induced　fluorescence　(LIF)　using　4-fluoro-7-nitro-2,1,3-benzoxadiazole　(NBD-F)　as　fluorogenic　reagent.　Peptide　antibiotics　were　derivatized　by　NBD-F　in　50　mmol/L　borate　buffer　(pH　7.　5)　for　45　min　at　60　°C.　The　fluorescence　derivatives　of　peptide　antibiotics　were　separated　on　a　packed　phenyl　capillary　column　with　a　mobile　phase　consisting　of　acetonitrile-potassium　phosphate　(pH　5.　0,　10　mmol/L)　(55　:45,　v/v)　at　the　flow　rate　of　0.　02　mL/min.　A　supplementary　pressure　of　3.　8　MPa　and　a　separation　voltage　of　-10　kV　were　applied.　The　limits　of　detection　(LODs,　S/N　=　3)　were　5.　0-10.　0　ng/mL,　which　fulfilled　the　requirement　of　maximum　residue　limits　for　examined　peptide　antibiotics.　The　method　was　applied　to　detect　peptide　antibiotics　in　milk　and　feed　stuffs.　The　spiked　recoveries　of　the　three　peptide　antibiotics　were　72.　9%　-112.　4%.　The　method　shows　some　advantages　on　the　simpler　pretreatment　and　higher　sensitivity,　which　can　be　of　great　benefit　to　the　residual　analysis　of　the　veterinary　drugs.　©　2010　Editorial　Office　of　Chinese　Journal　of　Chromatography.