A　novel　antioxidant　peptide　from　protein　hydrolysate　of　Allium　tuberosum　Rottler　was　identified　as　Phe-Pro-Leu-Pro-Ser-Phe　(FF-6).　It　could　not　only　quench　multiple　radicals　efficiently,　but　also　promote　cell　survival　by　protecting　against　oxidative　damage.　The　residue　Leu　played　a　vital　role　in　radical-scavenging　process　and　interaction　with　ABTS　[rad]+　radicals.　When　substituted　residue　Leu　with　Ala,　the　radical-scavenging　capacity　and　cytoprotective　effect　of　FF-6　were　almost　lost.　Moreover,　the　peptide　could　increase　the　expression　level　of　proteins　PI3K,　Akt,　phospho-Akt,　mTOR,　phospho-mTOR,　but　not　affect　peroxiredoxin-6.　According　to　these　results,　the　potential　mechanism　responsible　for　the　protection　of　oxidative　damage　and　promotion　of　cell　survival　was　taken　place　through　the　PI3K/Akt/mTOR　signaling　pathway.　These　findings　could　be　helpful　for　better　elucidating　the　antioxidant　mechanism　and　shed　light　on　synthetic　of　alternative　antioxidants　with　more　potent　activity　as　nutraceuticals　or　pharmaceuticals.　©　2017　Elsevier　Ltd