The　fabrication　of　nanomaterials-based　sensing　platform　has　attracted　a　great　deal　of　interest　due　to　their　unique　properties.　Here,　we　report　a　novel　hybrid　platform　of　g-C3N4　nanosheets/DNA-stabilized　Ag　nanoclusters　(CNNS/AgNCs)　for　sensing　application.　In　this　platform,　the　fluorescent　AgNCs　was　synthesized　using　a　pair　of　double-functional　ssDNA　sequence　as　a　template,　including　the　aptamer　segment　against　thrombin　and　C-rich　segment　for　AgNCs.　Next,　the　interaction　between　the　fluorescent　Apt-AgNCs　and　CNNS　was　investigated.　It　is　verified　that　DNA-stabilized　AgNCs　could　absorb　on　the　CNNS　surface　via　the　stronger　π−π　interaction　to　form　the　hybrid　platform,　whose　fluorescence　is　quenched　by　CNNS　through　the　photoelectron　transfer　effect　(PET).　When　targets　are　introduced　into　the　system,　target/Apt-AgNCs　complex　will　fall　off　from　the　CNNS　surface,　resulting　in　the　fluorescence　recovery.　This　hybrid　platform　can　achieve　the　detection　of　biomolecule　with　high　sensitivity　and　selectivity.　Considering　the　fluorescence　variability　of　DNA　scaffold　AgNCs,　this　hybrid　platform　is　promising　to　extend　to　other　target　and　even　multi-target　detection.　©　2019　Elsevier　B.V.