To　achieve　high　ionic　liquid　(IL)　tolerance　and　promote　in　situ　enzymatic　hydrolysis　of　biomass,　four　metal-organic　framework　(MOF)　carriers,　including　ZIF-8,　UIO-66-NH2,　MIL-100-Fe,　and　PCN-250,　were　used　for　cellulase　immobilization　through　simple　physical　adsorption.　The　results　showed　that　ZIF-8　had　the　largest　enzyme　adsorption　capacity　at　176.16　mg/g　support.　In　addition,　the　immobilized　cellulases　activity　was　evaluated　in　the　presence　of　1-ethyl-3-methylimidazolium　diethylphosphate　([Emim]DEP)　by　using　caryboxymethyl　cellulose　(CMC)　and　filter　paper　(FP)　as　substrates.　When　IL　concentration　increased　from　0%　to　50%　(v/v),　the　immobilized　cellulase　displayed　superior　IL　tolerance　compared　with　free　cellulase.　In　particular,　ZIF-8-immobilized　cellulase　exhibited　a　remarkable　IL　tolerance,　in　which　the　activity　of　caryboxymethyl　cellulase　(CMCase)　and　filter　paper　cellulase　(FPase)　were　increased　by　112.59%　and　59.86%　in　50%　(v/v)　[Emim]DEP,　respectively.　The　analysis　of　kinetic　parameters　further　suggested　that　the　immobilized　cellulase　had　a　lower　equilibrium　dissociation　constant　(kd)　value　and　a　higher　final　enzyme　plateau　activity　(ap)　value　in　a　reaction　system　involving　IL,　indicating　that　the　immobilized　enzyme　can　effectively　reduce　cellulase　inactivation　caused　by　IL.　Eventually,　ZIF-8-immobilized　cellulase　with　in　situ　hydrolysis　of　bagasse　increased　by　92.92%　compared　with　free　cellulase　in　50%　(v/v)　[Emim]DEP.　©　2019　American　Chemical　Society.