Adenosine　5′-triphosphate　(ATP),　as　a　“molecular　currency”　for　intracellular　energy　transfer,　not　only　participates　in　various　physiological　activities,　but　also　involves　in　various　pathological　processes　of　living　cells.　Therefore,　the　establishment　of　a　simple,　rapid,　sensitive　and　accurate　ATP　analysis　method　is　of　great　significance　for　the　study　of　its　physiological　functions.　In　this　work,　we　rationally　design　a　near-infrared　phenylboronic　acid　group-functionalized　dicyanovinyl　squaraine　(SQ-PBA3),　with　which　a　supramolecular　assembly　was　constructed　for　fluorescent　imaging　of　ATP.　In　PBS　buffer,　SQ-PBA3　changes　self-assembly　when　adding　cetyltrimethyl　ammonium　bromide　(CTAB)　deriving　fluorescence　response　of　SQ-PBA3　to　ATP　with　the　maximum　emission　at　700　nm,　due　to　the　specificity　of　phenylboronic　acid　to　diols　and　the　multiple　electrostatic　interactions　between　SQ-PBA3,　ATP　and　CTAB　molecules.　Meanwhile,　the　probe　was　successfully　applied　to　monitor　intracellular　ATP　level　in　MCF-7　cells　with　high　sensitivity　and　selectivity.　©　2019　Elsevier　Ltd