The　purpose　of　this　study　was　to　develop　a　collagen　(COL)　microspheres-based　steroidal　saponin　(SS)　formulation　which　was　expected　to　sustain　release　of　SS　with　high　encapsulation　efficiency.　The　microspheres　were　prepared　by　water-in-oil-in　water　double-emulsion　solvent　evaporation　technique　with　different　formulation　variables　such　as　concentration　of　COL　and　emulsification　time.　The　optimised　SS　microspheres　were　prepared　successfully　with　average　diameter　of　8.68 ± 0.34　μm,　encapsulation　efficiency　up　to　67.63 ± 1.31%,　and　integrated　spherical　shape　with　no　drug　crystal　and　porous　on　its　surface.　The　in-vitro　drug　release　studies　showed　marked　retardation　of　SS　release　and　higher　percentage　of　COL　gave　longer　drug　release　profile.　Higuchi　model　was　found　to　be　the　best　fitted　for　drug　release　from　COL　microspheres　of　all　formulations.　This　may　suggest　the　potential　use　of　SS　microspheres　for　treatment　of　bone　tissue　engineering　over　long　periods.　©　2019,　©　2019　Informa　UK　Limited,　trading　as　Taylor　&　Francis　Group.