Wound　dressings　with　long-term　antimicrobial　activity　are　highly　desired　for　treatment　of　chronic　wound　infections.　Herein,　the　sustained　antimicrobial　wound　dressings　were　developed　by　using　antibiotic　agents,　ciprofloxacin　HCL　(CIP)　and　gentamicin　sulfate　(GS),　covalent　bonding　to　natural　polymer　matrix　composites,　carboxymethyl　chitosan　(CMC)　and　collagen　(COL).　By　amide　bond　formation　between　antibiotic　agents　and　polymer　chains,　two　antimicrobial　wound　dressings　CMC-COL-CIP　and　CMC-COL-GS　were　prepared.　The　presented　wound　dressings　exhibited　high　water　absorption　capacity,　excellent　water　vapor　transmission　rate　(WVTR),　appropriate　mechanical　properties,　and　impressive　stability.　Cytocompatibility　of　the　dressings　was　demonstrated　by　in　vitro　human　skin　fibroblast　(HSF)　cells　culture　study.　The　results　of　in　vitro　and　in　vivo　studies　indicated　that　the　two　antimicrobial　wound　dressings　have　effective　antimicrobial　activity　and　prolonged　antimicrobial　period.　Furthermore,　the　antimicrobial　dressings　could　promote　the　wound　healing,　reepithelialization,　collagen　deposition,　and　angiogenesis.　It　also　displays　superiority　wound　healing　effects　compared　to　commercially　available　silver-based　dressings　(Aguacel　Ag).　This　work　indicates　that　the　prepared　antimicrobial　wound　dressings　have　great　potential　application　in　chronic　wound　healing,　such　as　severe　wound　cure　and　diabetic　foot　ulcers.　©　2018　Elsevier　B.V.