Based　on　our　developed　sodium　hyaluronate-functionalized　urea-formaldehyde　(HA-UF)　monolith,　a　silver　nanoparticles-coated　monolithic　column　has　been　fabricated　via　the　interaction　between　silver　nanoparticles　(Ag　NPs)　and　HA.　The　successful　coating　of　Ag　NPs　on　the　parent　monolith　was　proven　by　SEM,　EDAX,　UV–vis　spectrum　and　XPS.　Nitrogen　adsorption　desorption　isotherms　and　Barret-Joyner-Halenda　(BJH)　pore　size　distributions　of　the　parent　and　resultant　monolith　were　also　performed.　Due　to　the　Ag+-like　affinity　interaction　caused　by　the　contact　of　unsaturated　compounds　and　Ag　NPs,　the　Ag　NPs-coated　monolith　showed　satisfactory　extraction　efficiency　towards　these　compounds,　and　was　applied　for　in-tube　solid-phase　microextraction　(SPME)　of　monounsaturated　fatty　acid　methyl　esters　(MUFAMEs).　Several　factors　for　in-tube　SPME,　such　as　ACN　percentage　in　the　sampling　solution,　elution　volume,　sampling　and　elution　flow　rate,　were　investigated　with　respect　to　the　extraction　efficiency　of　model　MUFAMEs.　Under　the　optimized　SPME　conditions,　a　simple　in　tube　SPME-Ag+-HPLC　method　for　detection　of　model　MUFAMEs　has　been　proposed,　the　limits　of　detection　(LODs)　were　less　than　5.2　μg/kg,　and　the　recoveries　of　spiked　French　fry　samples　were　ranged　from　86.6%　to　96.1%　with　relative　standard　deviations　(RSDs)　less　than　5.2%.　This　study　provided　an　Ag　NPs-coated　monolith　with　good　reproducibility　and　repeatable　extraction　performance,　and　developed　an　efficient　method　for　in-tube　SPME　of　MUFAMEs　in　practical　food　samples.　©　2018　Elsevier　B.V.