Microalgae　have　great　potential　for　both　total　nitrogen/phosphorus　(TN/TP)　removal　and　Cu(II)　biosorption.　The　objective　of　this　study　was　to　optimize　the　biofilm　production　and　TN/TP/Cu(II)　removal　with　attached　culture　of　Chlamydomonas　sp.　JSC4　in　swine　wastewater　using　an　innovative　fixed-bed　biofilm　reactor　(FBR).　The　influences　of　medium　volume,　N/P　ratios　and　Cu(II)　concentration　were　investigated.　The　maximum　biofilm　productivity　of　49.70　g　m−2　d−1　was　obtained　with　300　mL　original　wastewater　(TN:　600　mg　L−1;　N/P　ratio:　23:1;　Cu(II):　0.23　mg　L−1),　which　was　1.875　L　wastewaer　for　1　m2　of　cultivation　surface.　Although　the　passive　adsorption　of　Cu(II)　greatly　inhibited　the　adsorption　of　TN/TP,　detoxification　associated　with　active　metabolism　was　conducive　to　promoting　the　removal　of　TN/TP.　A　high　Cu(II)　concentration　of　15　mg　L−1　was　tolerated,　under　which,　the　biofilm　productivity　of　37.73　g　m−2　d−1,　Cu(II)　removal　capacity　of　3.10　mg　g−1　and　TN/TP/　Cu(II)　removal　efficiency　of　85.79%/96.56%93.70%　were　achieved,　respectively.　©　2018　Elsevier　B.V.