This　is　the　first　time　that　two-dimensional　(2D)　amorphous　MoS3　nanosheets　were　prepared　by　the　exfoliation　of　the　bulk　amorphous　MoS3　material　in　dimethylformamide　(DMF)　solvent　under　ultrasonic　irradiation.　The　obtained　2D　amorphous　MoS3　nanosheets　show　a　unique　porous　network　structure,　which　is　quite　different　from　that　of　2D　crystalline　MoS2　nanosheets.　The　XPS　and　Raman　results　reveal　that　the　structure　of　2D　amorphous　MoS3　nanosheets　contains　high　levels　of　active　sulphide　groups　with　unsaturated　bonds,　suggesting　that　this　material　can　be　applied　as　an　efficient　heavy　metal　scavenger　in　the　field　of　environmental　remediation.　The　interaction　mechanisms　between　metal　ions　and　specific　sulphide　groups　on　2D　amorphous　MoS3　nanosheets　have　been　discussed.　The　feasibility　of　toxic　metal　remediation　(Cu,　Cd　and　Hg)　from　a　synthetic　acid　mine　drainage　solution　has　been　proven.　The　results　suggest　that　2D　amorphous　MoS3　nanosheets　as　a　new　member　of　the　2D　material　family　will　have　promising　applications　in　environmental　protection.　©　2019　The　Royal　Society　of　Chemistry.