Objective:　In　order　to　improve　the　digestive　bioaccessibility　of　bamboo　leaf　flavonoids,　this　study　aimed　to　clarify　the　protective　effect　of　pectin　on　bamboo　leaf　flavonoids　during　simulated　in　vitro　gastrointestinal　digestion.　Methods:　The　concentrations　of　isoorientin,　orientin,　isovitexin　and　vitexin　from　bamboo　leaves　at　different　stages　of　digestion　in　the　presence　and　absence　of　pectin　were　determined　by　ultra-high　performance　liquid　chromatography　(UPLC).　Results:　Compared　with　the　undigested　samples,　direct　digestion　(without　pectin)　significantly　decrease　in　the　concentrations　of　the　four　C-glycosylflavones　(P　＜　0.05).　Moreover,　oral,　gastric　and　intestinal　digestion　had　different　effects　on　the　flavonoid　concentrations.　It　was　highlighted　that　the　bioaccessibility　of　these　flavonoids　was　significantly　improved　by　pectin　treatment　(P　＜　0.05).　Especially,　the　bioaccessibility　of　isovitexin　and　vitexin　was　increased　by　75.1%　and　80.2%,　respectively.　Moreover,　principal　component　analysis　and　cluster　analysis　demonstrated　different　cluster　tendencies　for　the　four　bamboo　leaf　flavonoids　after　treatments.　Conclusion:　Pectin　is　useful　to　enhance　the　bioaccessibility　of　bamboo　leaf　C-glycosylflavones,　indicating　its　promising　application　in　functional　food　products　containing　these　flavonoid　compounds.　©　2020,　China　Food　Publishing　Company.　All　right　reserved.