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In this study, ten typical Hong Qu samples, including five GuTian Qu and five WuYi Qu, were collected for the analysis of the fungal structure, by utilizing culture-independent molecular biological methods. Through the PCR-DGGE analysis, it was revealed that, compared with GuTian Qu, WuYi Qu had higher species richness, diversity and evenness. Moreover, via Principal Component Analysis and Cluster Analysis, it was found that there was significant difference of fungal structure between these two types of Hong Qu. The predominant fungi in the starters, including Monascus purpureus, Aspergillus flavus, Rhizopus oryzae, Saccharomyces cerevisiae, were also enumerated with Real-Time PCR approach. It was determined that, M. purpureus counts in GuTian Qu and WuYi Qu were (6.07 ± 0.25) lg (CFU/g) and (5.82 ± 0.11) lg (CFU/g), respectively, which without significant difference (P>0.05). However, dramatic difference of the populations of A. flavus, R. oryzae, S. cerevisiae in these two types of Hong Qu were found (P<0.05). For GuTian Qu, the populations of A. flavus, R. oryzae and S. cerevisiae were (5.94 ± 1.04) lg (CFU/g), (4.65 ± 0.91) lg (CFU/g) and (3.33 ± 0.99) lg (CFU/g), respectively. For WuYi Qu, the populations of A. flavus, R. oryzae and S. cerevisiae were (8.21 ± 1.22) lg (CFU/g), (6.71 ± 1.27) lg (CFU/g) and (5.41 ± 0.44) lg (CFU/g), respectively. In conclusion, Hong Qu have a complex fungal diversity, with M. purpureus as the most predominant fungi. This study provided a detailed insight into the fungal community of Hong Qu. © 2019, Editorial Office of Journal of CIFST. All right reserved.
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Journal of Chinese Institute of Food Science and Technology
ISSN: 1009-7848
Year: 2019
Issue: 1
Volume: 19
Page: 200-206
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ESI Highly Cited Papers on the List: 0 Unfold All
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30 Days PV: 2
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