Methods for mammalian single cell research - a review [哺乳动物单细胞研究技术的现状与未来] - Details

author：

Jiang, W. (Jiang, W..) ^[1] | Tian, Y. (Tian, Y..) ^[2] | Zuo, R. (Zuo, R..) ^[3] | Lin, J. (Lin, J..) ^[4]

Indexed by：

Scopus PKU CSCD

Abstract：

Basic　research　in　life　science　and　medicine　has　dug　into　single　cell　level　in　recent　years.　Single-cell　analysis　offers　to　understand　life　from　diverse　perspectives　and　is　used　to　profile　cell　heterogeneity　to　investigate　mechanism　of　diseases.　Single　cell　technologies　have　also　found　applications　in　forensic　medicine　and　clinical　reproductive　medicine,　while　the　techniques　are　rapidly　evolving　and　have　become　more　and　more　sophisticated.　In　this　article,　we　reviewed　various　single　cell　isolation　techniques　and　their　pros　and　cons,　including　manual　cell　picking,　laser　capture　microdissection　and　microfluidics,　as　well　as　analysis　methods　for　DNA,　RNA　and　protein　in　single　cell.　In　addition,　we　summarized　major　up-to-date　single　cell　research　achievements　and　their　potential　applications.　©　2019,　Science　Press.　All　right　reserved.

Keyword：

Laser capture microdissection (LCM); Microfluidic; Single cell; Single cell genome sequencing; Single cell transcriptome sequencing

Community：

[ 1 ] [Jiang, W.]Institute of Applied Genomics, Fuzhou University, Fuzhou, Fujian 350108, China
[ 2 ] [Jiang, W.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China
[ 3 ] [Tian, Y.]Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, Gothenburg, 40530, Sweden
[ 4 ] [Zuo, R.]Institute of Applied Genomics, Fuzhou University, Fuzhou, Fujian 350108, China
[ 5 ] [Zuo, R.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China
[ 6 ] [Lin, J.]Institute of Applied Genomics, Fuzhou University, Fuzhou, Fujian 350108, China
[ 7 ] [Lin, J.]School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350122, China
[ 8 ] [Lin, J.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China

Reprint 's Address：

[Lin, J.]Institute of Applied Genomics, Fuzhou UniversityChina

Email：

jun@fzu.edu.cn

Show more details

Related Keywords：

Source ：

Chinese Journal of Biotechnology

ISSN： 1000-3061

Year： 2019

Issue： 1

Volume： 35

Page： 27-39

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 1

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 0

Affiliated Colleges：

Get Fulltext

DOI Library Discovery Baidu Scholar Search SCOPUS

Type
Departments

All Years Choose Year From to