Preparation recombination human-like collagen/fibroin scaffold and promoting the cell compatibility with osteoblasts - Details

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Abstract：

On　this　basis,　a　novel　recombinant　human-like　collagen　(RHLC)/silk　fibroin　scaffold　material　with　high　porosity　and　controllable　aperture　was　prepared.　The　compatibility　of　osteoblasts　(OB)　with　the　blends　was　tested　in　vitro.　The　morphology,　adhesion　and　growth　of　scaffold　cells　were　observed　by　scanning　electron　microscope　and　laser　confocal　microscope.　Extensive　measurements,　including　3-[4,　5-dimethylthiazole-2-acyl]-2,　5-diphenyl　tetrabrominate　assays,　intracellular　total　protein　content,　and　alkaline　phosphatase　activity　assays　were　performed　after　7　days　of　culture.　Survival　and　protein　content　increased　in　RHLC/fibroin　stents.　LSCM　and　SEM　results　confirmed　that　the　cells　grew　better　in　the　mixed　scaffolds　than　in　the　pure　silk　scaffolds,　and　showed　that　the　cells　were　easy　to　adhere　and　diffuse　in　the　RHLC/silk　scaffolds.　RHLC/silk　fibroin　scaffolds　are　promising　biomaterials　for　bone　tissue　engineering.　©　2020　Wiley　Periodicals　LLC

Keyword：

biomaterial; fibroin; osteoblasts; RHLC; scaffold

Community：

[ 1 ] [Hu, K.]Beijing Engineering Research Center of Printed Electronics, Institute of Printing and Packaging Engineering, Beijing Institute of Graphic Communication, Beijing, China
[ 2 ] [Hu, K.]College of Biological Science and Engineering, Fuzhou University, Fuzhou, China
[ 3 ] [Hu, M.]Beijing Engineering Research Center of Printed Electronics, Institute of Printing and Packaging Engineering, Beijing Institute of Graphic Communication, Beijing, China
[ 4 ] [Xiao, Y.]Beijing Engineering Research Center of Printed Electronics, Institute of Printing and Packaging Engineering, Beijing Institute of Graphic Communication, Beijing, China
[ 5 ] [Xiao, Y.]School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, China
[ 6 ] [Cui, Y.]Beijing Engineering Research Center of Printed Electronics, Institute of Printing and Packaging Engineering, Beijing Institute of Graphic Communication, Beijing, China
[ 7 ] [Yan, J.]Beijing Engineering Research Center of Printed Electronics, Institute of Printing and Packaging Engineering, Beijing Institute of Graphic Communication, Beijing, China
[ 8 ] [Yang, G.]Beijing Engineering Research Center of Printed Electronics, Institute of Printing and Packaging Engineering, Beijing Institute of Graphic Communication, Beijing, China
[ 9 ] [Zhang, F.]Department of Neurosurgery, The First Hospital of Fuzhou Medical Association, Fuzhou, China
[ 10 ] [Lin, G.]Technology Department, FuJian HuaRui Biological Technology Co., Ltd, Fuzhou, China
[ 11 ] [Yi, H.]Technology Department, Tsinghua Redbud Innovation Institute Baodi Tianjin, Tianjin, China
[ 12 ] [Han, L.]Beijing Engineering Research Center of Printed Electronics, Institute of Printing and Packaging Engineering, Beijing Institute of Graphic Communication, Beijing, China
[ 13 ] [Li, L.]Beijing Engineering Research Center of Printed Electronics, Institute of Printing and Packaging Engineering, Beijing Institute of Graphic Communication, Beijing, China
[ 14 ] [Wei, Y.]Department of Chemistry and Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing, China
[ 15 ] [Cui, F.]School of Materials Science and Engineering, Tsinghua University, Beijing, China

Reprint 's Address：

[Hu, K.]Beijing Engineering Research Center of Printed Electronics, Institute of Printing and Packaging Engineering, Beijing Institute of Graphic Communication, College of Biological Science and Engineering, Fuzhou University, Department of Neurosurgery, The First Hospital of Fuzhou Medical AssociationChina

Email：

kunhu@139.com

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Source ：

Journal of Biomedical Materials Research - Part A

ISSN： 1549-3296

Year： 2020

4 . 3 9 6

JCR@2020

3 . 9 0 0

JCR@2023

ESI HC Threshold：196

JCR Journal Grade：2

CAS Journal Grade：3

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 14

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 2

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