The　speciation　analysis　of　arsenic　in　seafood　is　of　great　significance　for　objectively　and　scientifically　assessing　arsenic　health　risks　in　seafood.　In　general,　different　types　of　seafood　contain　a　variety　of　different　arsenic　species　and　have　quite　different　matrix.　Thus,　the　detection　of　arsenic　species　in　different　types　of　seafood　required　different　extracting　and　analytical　methods.　In　this　study,　a　universal　microwave-assisted　extraction　was　developed　to　extract　each　arsenic　species　including　As(V),　As(III),　monomethylarsonic　acid　(MMA),　dimethylarsinic　acid　(DMA),　arsenobetaine　(AsB),　arsenosugars　and　so　on　in　various　seafood　with　a　extraction　efficiency　＞　95%　and　without　altering　their　original　species,　and　a　universal　cation　exchange　IC-ICP-MS　(ion　chromatography-inductively　coupled　plasma　mass　spectrometry)　method　was　developed　for　the　detection　of　arsenic　species　in　various　seafood　(seaweed,　fish,　shellfish　and　shrimp)　with　a　method　detection　limit　of　8.0　ng　As/g　dried　seafood–12.0　ng　As/g　dried　seafood.　Especially,　the　extract　of　seafood　obtained　with　our　microwave-assisted　extraction　can　be　directly　used　for　the　following　IC-ICP-MS　detection　without　any　additional　pretreatment.　By　using　the　extraction　method　and　IC-ICP-MS　method,　we　have　successfully　detected　each　arsenic　species　including　As(V),　As(III),　MMA,　DMA,　AsB,　arsenosugars　and　so　on　in　seaweed,　fish,　shellfish　and　shrimp　samples　with　a　recovery　of　92%–104%　and　a　relative　standard　deviation　(RSD,　n　=　5)　＜　5%.　The　developed　microwave-assisted　extraction　method　and　IC-ICP-MS　detection　method　is　simple,　has　broad　applicability,　high　efficiency,　excellent　accuracy　and　strong　resistibility　to　the　complicated　matrix,　which　promising　a　reliable　approach　for　objectively　and　scientifically　assessing　arsenic　health　risks　in　various　seafood　to　ensure　the　consumption　safety　of　seafood.　©　2020　Elsevier　B.V.