In　this　study,　a　double-recognized　aptamer-molecularly　imprinted　monolithic　column　(Apt-MIP　monolithic　column)　was　prepared　by　introducing　both　aptamer　and　MIP　to　reduce　non-specific　adsorption.　Its　preparation　parameters　such　as　the　time　of　photo-initiation,　the　dosage　of　photo-initiator　and　the　concentration　of　aptamer　were　investigated　in　detail.　The　recovery　ratios　of　ochratoxin　A　(OTA)　to　ochratoxin　B　(OTB)　on　Apt-MIP　monolithic　column,　Apt　monolithic　column　and　MIP　monolithic　column　were　116.1,　40.8　and　69,　respectively.　Even　if　the　concentration　of　OTB　was　10　times　that　of　OTA,　the　recovery　of　OTB　was　only　about　2.9%.　Applied　to　beer　samples,　the　prepared　Apt-MIP　monolithic　column　drastically　resisted　background　adsorption　and　the　high-specificity　recognition　for　OTA　was　obtained　with　the　recoveries　of　95.5–105.9%.　This　work　provided　a　simple　and　effective　method　to　selectively　identify　OTA　from　complex　samples.　©　2019　Elsevier　B.V.