A　specific　electrochemical　immunosensor　for　ultra-sensitive　determination　of　Aflatoxin　B1　(AFB1)　was　developed　according　to　a　dual　enhancing　strategy.　The　dual-doped　graphene　(BS-G)　was　obtianed　by　a　simple　microwave-assisted　hydrothermal　approach,　sulfur　and　boron　affinity　for　peanut-shaped　Au@Pt　NRs　embedded　into　BS-G　nanosheets,　ultrathin　BS-G　effectively　enwrapped　most　of　the　individual　Au@Pt　NRs　with　excellent　dispersibility.　It　is　noteworthy　that　two　faces　at　the　interface　between　(002)　crystal　faces　of　BS-G　and　(111)　planes　of　Au@Pt　NRs　are　compatible,　accelerating　the　charge　transfer　between　the　hybrid　nanocatalysts　and　AFB1.　Moreover,　the　highly　dispersed　COOH　groups　and　defect　sites　of　the　BS-G　precisely　control　the　orientation　of　anti-AFB1　immobilized　on　the　sensor　surface,　meanwhile,　the　strong　electronic　interactions　endow　the　Au@Pt　NRs/BS-G　hybrid　nanocatalysts　with　remarkable　biocompatibility　and　multivalent　affinity　interactions　with　the　high　molar　ratio　of　anti-AFB1　for　enhanced　immunoreactions.　Thus,　the　designed　immunosensor　exhibits　ultra-efficient　electron-transfer　abilities,　excellent　sensitivity,　and　favorable　selectivity,　the　detection　limit　is　0.0052　ng·mL-1　and　the　linear　range　is　0.025-60.00　ng·mL-1.　©　2020　The　Authors.