Antioxidants inhibit cell senescence and preserve stemness of adipose tissue-derived stem cells by reducing ROS generation during long-term in vitro expansion - Details

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Abstract：

Background:　Adipose　tissue-derived　mesenchymal　stem　cells　(ADSCs)　are　promising　candidates　for　regenerative　medicine.　However,　long-term　in　vitro　passaging　leads　to　stemness　loss　and　cell　senescence　of　ADSCs,　resulting　in　failure　of　ADSC-based　therapy.　Methods:　In　this　study,　ADSCs　were　treated　with　low　dose　of　antioxidants　(reduced　glutathione　and　melatonin)　with　anti-aging　and　stem　cell　protection　properties　in　the　in　vitro　passaging,　and　the　cell　functions　including　stem　cell　senescence,　cell　migration,　cell　multidirectional　differentiation　potential,　and　ROS　content　were　carefully　analyzed.　Results:　We　found　that　GSH　and　melatonin　could　maintain　ADSC　cell　functions　through　reducing　cell　senescence　and　promoting　cell　migration,　as　well　as　by　preserving　stemness　and　multidirectional　differentiation　potential,　through　inhibiting　ROS　generation　during　long-term　expansion　of　ADSCs.　Conclusions:　Our　results　suggested　that　antioxidant　treatment　could　efficiently　prevent　the　dysfunction　and　preserve　cell　functions　of　ADSCs　after　long-term　passaging,　providing　a　practical　strategy　to　facilitate　ADSC-based　therapy.　©　2019　The　Author(s).

Keyword：

Adipose tissue-derived mesenchymal stem cells; Melatonin; Reduced glutathione; ROS; Senescence; Stemness

Community：

[ 1 ] [Liao, N.]United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 2 ] [Liao, N.]Liver Disease Center, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350007, China
[ 3 ] [Liao, N.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 4 ] [Shi, Y.]United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 5 ] [Shi, Y.]Liver Disease Center, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350007, China
[ 6 ] [Shi, Y.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 7 ] [Zhang, C.]United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 8 ] [Zhang, C.]Liver Disease Center, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350007, China
[ 9 ] [Zhang, C.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 10 ] [Zheng, Y.]United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 11 ] [Zheng, Y.]Liver Disease Center, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350007, China
[ 12 ] [Zheng, Y.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 13 ] [Wang, Y.]United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 14 ] [Wang, Y.]Liver Disease Center, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350007, China
[ 15 ] [Wang, Y.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 16 ] [Zhao, B.]United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 17 ] [Zhao, B.]Liver Disease Center, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350007, China
[ 18 ] [Zhao, B.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 19 ] [Zeng, Y.]United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 20 ] [Zeng, Y.]Liver Disease Center, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350007, China
[ 21 ] [Zeng, Y.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 22 ] [Zeng, Y.]Liver Center of Fujian Province, Fujian Medical University, Fuzhou, 350025, China
[ 23 ] [Liu, X.]United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 24 ] [Liu, X.]Liver Disease Center, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350007, China
[ 25 ] [Liu, X.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 26 ] [Liu, J.]United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, China
[ 27 ] [Liu, J.]Liver Disease Center, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350007, China
[ 28 ] [Liu, J.]Mengchao Med-X Center, Fuzhou University, Fuzhou, 350116, China
[ 29 ] [Liu, J.]Liver Center of Fujian Province, Fujian Medical University, Fuzhou, 350025, China

Reprint 's Address：

[Liu, X.]United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical UniversityChina

Email：

xiaoloong.liu@gmail.com

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Source ：

Stem Cell Research and Therapy

ISSN： 1757-6512

Year： 2019

Issue： 1

Volume： 10

4 . 6 2 7

JCR@2018

CAS Journal Grade：2

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WoS CC Cited Count：

SCOPUS Cited Count： 72

ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 0

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