Amphibians　are　a　natural　source　of　abundant　antimicrobial　peptides　and　thus　have　been　widely　investigated　for　isolation　of　such　biomolecules.　Many　new　antimicrobial　peptide　families　have　been　discovered　from　amphibians.　In　this　study,　a　novel　antimicrobial　peptide　named　Limnonectes　fujianensis　Brevinvin　(LFB)　has　been　identified　in　the　skin　secretion　from　the　Fujian　large　headed　frog,　Limnonectes　fujianensis.　The　cDNA　sequence　was　cloned　from　a　skin　secretion　library　and　the　predicted　mature　peptide　was　identified　through　MS/MS　fragmentation　sequencing　of　reverse　phase　HPLC　fractions　on　the　same　sample.　LFB　was　predicted　to　be　an　amphipathic,　hydrophobic,　alpha　helical,　and　beta　turn　peptide　that　inserts　into　a　lipid　bilayer　in　order　to　kill　the　cells.　In　antimicrobial　assays,　a　synthetic　replicate　of　this　novel　antimicrobial　peptide　demonstrated　significant　activity　against　the　Gram-positive　bacterium　Staphylococcus　aureus,　the　Gram-negative　bacterium　Escherichia　coli　and　the　yeast,　Candida　albicans.　This　novel　peptide　was　highly　potent　(MIC　4.88　uM)　against　Gram-negative　bacterium,　and　also　has　the　ability　to　inhibit　the　growth　of　human　cancer　cell　lines　with　IC50　values　ranging　from　18.9　µM　down　to　2.0　µM.　These　findings　help　to　enrich　our　understanding　of　Brevinin-like　peptides.　Moreover,　the　data　presented　here　validate　frog　secretion　as　a　source　of　potential　novel　antimicrobial　peptides,　that　also　exhibit　anti-tumor　properties,　that　could　be　useful　for　the　treatment　of　cancer.　©　2019　by　the　authors.　Licensee　MDPI,　Basel,　Switzerland.