A　competitive　fluorescence　immunoassay　for　the　identification　and　quantification　of　morphine　has　been　developed　on　the　basis　of　hapten-coated　plate　format.　Hapten　was　prepared　through　covalent　conjugating　a　morphine　derivative　with　albumin　bovine.　In　the　immunoassay,　the　hapten　was　inoculated　on　a　96-well　plate　and　then　bound　with　monoclonal　antibodies　labeled　with　a　signal　indicating　dye,　fluorescein　isothiocyanate　(FITC).　Unbound　FITC-antibodies　were　rinsed　off　from　the　plate.　The　fluorescein　intensity　decreases　in　the　presence　of　morphine　molecules　due　to　the　competitively　binding　to　antibodies　against　hapten.　The　intensity　is　inversely　correlated　with　the　concentration　of　morphine.　In　quantitative　analysis　for　urine　samples,　we　obtained　a　linearity　range　of　0.2　μg/mL∼2.5　μg/mL,　along　with　a　detection　limit　of　c.a.　1　ng/mL.　The　fluorescence　immunoassay　shows　low　cross-reactivity　(below　10%)　to　6-acetylmorphine,　3-acetylmorphine,　and　heroine.　The　developed　method　produced　comparable　results　to　the　standard　GC-MS/MS　method.　In　conclusion,　a　rapid　and　efficient　screening　tool　for　morphine　in　clinical　human　urine　has　been　established.　©　2019　Jie　Cao　et　al.