A　novel　form　of　reversed-phase　liquid　chromatography　(RPLC)　by　the　dynamically　modified　hydrophilic　interaction　monolithic　column　has　been　described　in　this　paper.　A　porous　poly(SPMA-co-PETA)　monolith　with　strong　cation-exchange　(SCX)　was　prepared　and　the　resulting　monolith　showed　a　typical　hydrophilic　interaction　chromatography　(HILIC)　mechanism　at　higher　organic　solvent　content　(ACN%　＞　50%).　The　good　selectivity　for　neutral,　basic　and　acidic　polar　analytes　was　observed　in　the　HILIC　mode.　In　order　to　increase　the　hydrophobic　interaction,　the　monolith　with　SCX　was　dynamically　modified　with　a　long-chain　quaternary　ammonium　salt,　cetyltrimethylammonium　bromide　(CTAB),　which　was　added　to　the　mobile　phase.　CTAB　ions　were　adsorbed　onto　the　surface　of　the　SCX　monolithic　material,　and　the　resulting　hydrophobic　layer　was　used　as　the　stationary　phase.　Using　the　dynamically　modified　SCX　monolithic　column,　neutral,　basic　and　acidic　hydrophobic　analytes　were　well　separated　with　the　RPLC　mode.　Crown　Copyright　©　2009.