An electrochemical biosensor for ultratrace terbium based on Tb3+ promoted conformational change of human telomeric G-quadruplex - Details

author：

Zhang, J. (Zhang, J..) ^[1] | Chen, J. (Chen, J..) ^[2] | Chen, R. (Chen, R..) ^[3] | Chen, G. (Chen, G..) ^[4] | Fu, F. (Fu, F..) ^[5]

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Abstract：

A　new　electrochemical　biosensor　for　the　monitoring　of　ultratrace　terbium　based　on　the　conformational　change　of　DNA　containing　a　single　guanine　(G)-rich　stretch　was　described　here.　The　biosensor　was　fabricated　by　immobilizing　a　thiolated　DNA　containing　a　single　G-rich　stretch　on　the　gold　surface　as　probe　surface.　The　G-rich　DNA　probe　was　found　to　be　capable　of　changing　its　configuration　from　flexible　single-stranded　structures　to　rigid　tetramolecular　G-quadruplex　in　the　presence　of　terbium　III,　which　provided　a　switchable　charge　transport　path　for　the　oxidation　of　[Fe(CN)6]4-.　The　switchable　surface　provided　a　sensing　platform　for　the　single-step　and　reagentless　detection　of　Tb3+.　Using　this　reusable　electrochemical　sensing　platform,　a　simple,　rapid,　and　selective　biosensor　for　the　determination　of　ultratrace　terbium　ions　with　a　detection　limit　of　6.0　×　10-11　M　has　been　developed.　The　success　in　the　present　biosensor　served　as　a　significant　step　toward　the　development　of　monitoring　ultratrace　Tb3+　in　river　water　or　seawater.　©　2009　Elsevier　B.V.　All　rights　reserved.

Keyword：

Electrochemical biosensor; G-quadruplex; Human telomeric; Reversible switch; Terbium

Community：

[ 1 ] [Zhang, J.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 2 ] [Zhang, J.]Pharmaceutical Department, Fujian College of Medical Occupation and Technology, Fuzhou, 350101, China
[ 3 ] [Chen, J.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 4 ] [Chen, J.]Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, 350004, China
[ 5 ] [Chen, R.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 6 ] [Chen, G.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 7 ] [Fu, F.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China

Reprint 's Address：

[Fu, F.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China

Email：

fengfu@fzu.edu.cn

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Source ：

Biosensors and Bioelectronics

ISSN： 0956-5663

Year： 2009

Issue： 2

Volume： 25

Page： 378-382

5 . 4 2 9

JCR@2009

1 0 . 7 0 0

JCR@2023

JCR Journal Grade：1

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