A　method　involving　microwave　digestion　and　inductively　coupled　plasma-mass　spectrometric　(ICP-MS)　analysis　was　established　for　the　determination　of　five　elements　(Cu,　As,　Cd,　Hg,　Pb)　in　Venenum　Bufonis.　Detection　limits　for　these　five　elements　were　in　the　range　of　0.28　μ/L　to　5.7　μg/L,　and　the　recoveries　ranged　from　91.5%　to　115%.　The　arsenic　species　in　venenum　bufonis　were　extracted　using　1.2　mol/L　hydrochloric　acid,　in　addition,　the　chemical　forms　of　arsenic　including　inorganic　As(V)　in　Venenum　Bufonis　samples　were　quantified　by　the　hyphenated　technique　of　high　performance　liquid　chromatography　coupled　with　inductively　coupled　plasma-mass　spectrometry　(HPLC-ICP-MS).　The　speciation　results　are　presented　and　the　analytical　merits　of　the　technique　are　discussed.　The　technique　was　applied　for　the　quality　control　and　safety　evaluation　of　Venenum　Bufonis　samples.