Enamel　cannot　heal　by　itself　if　damaged.　Hydroxyapatite　(HAP)　is　main　component　of　human　enamel.　Formation　of　enamel-like　materials　for　healing　enamel　defects　remains　a　challenge.　In　this　paper,　we　successfully　isolated　the　abalone　water-soluble　matrix　(AWSM)　with　1.53　wt%　the　abalone　water-soluble　protein　(AWSPro)　and　2.04　wt%　the　abalone　water-soluble　polysaccharide　(AWSPs)　from　abandoned　abalone　shell,　and　self-healing　biomineralization　of　tooth　defects　was　successfully　achieved　in　vitro.　Based　on　X-ray　diffraction　(XRD),　Fourier　transform　infrared　spectroscopy　(FTIR),　hot　field　emission　scanning　electron　microscopy　(HFESEM)　and　energy　dispersive　spectrometer　(EDS)　analysis,　the　results　showed　that　the　AWSM　can　efficiently　induce　remineralization　of　HAP.　The　enamel-like　HAP　was　successfully　achieved　onto　etched　enamel＇s　surface　due　to　the　presence　of　the　AWSM.　Moreover,　the　remineralized　effect　of　eroded　enamel　was　growing　with　the　increase　of　the　AWSM.　This　study　provides　a　solution　to　the　resource　waste　and　environmental　pollution　caused　by　abandoned　abalone　shell,　and　we　provides　a　new　method　for　self-healing　remineralization　of　enamel　defects　by　AWSM　and　develops　a　novel　dental　material　for　potential　clinical　dentistry　application.　©　2016　Elsevier　B.V.　All　rights　reserved.