Surfactant　adsorption　films　at　the　air/water　interface,　which　usually　exert　a　significant　influence　over　system　behaviour,　are　closely　related　to　the　surfactant　molecular　structure.　In　this　research,　a　series　of　Gemini　surfactants　containing　four　hydroxyl　groups　at　the　head　groups,　namely:　alkanediyl-α,ω-bis[di(2-hydroxylethyl)　dodecylammonium　bromide],　abbreviated　to　12(2OH)-s-12(2OH),　where　s　=　3,　6,　8　and　10,　respectively,　were　investigated　by　using　interfacial　dilatational　rheological　measurements.　The　effects　of　oscillating　frequency,　bulk　concentration　and　spacer　length　on　the　dilatational　behaviour　of　12(2OH)-s-12(2OH)　were　investigated.　The　variation　of　dilatational　modulus　of　12(2OH)-8-12(2OH)　with　concentrations　showed　two　peaks　which　were　attributed　to　different　configurations　adopted　by　the　spacer,　revealing　the　effects　of　dynamic　configuration　of　the　spacer　on　surfactant　interfacial　adsorption.　The　fit　to　the　results,　with　that　data　available,　using　the　Lucassen/van　den　Tempel　(LVT)　model　showed　that　the　presence　of　hydroxyl　groups　near　the　head　groups　of　Gemini　surfactants　enhanced　the　interfacial　elasticity　of　the　adsorption　films.　©　2016,　Springer-Verlag　Berlin　Heidelberg.