• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

Wu, J. (Wu, J..) [1] | Wang, S. (Wang, S..) [2] | Sun, X. (Sun, X..) [3] | Cao, M. (Cao, M..) [4] | Chen, M. (Chen, M..) [5] | Wang, J. (Wang, J..) [6] | Zhang, Q. (Zhang, Q..) [7]

Indexed by:

Scopus

Abstract:

A 72-kDa matrix metalloproteinase (MMP; sMP) was purified from silver carp skeletal muscle by 30-70% ammonium sulfate fractionation and chromatography. The sMP revealed high gelatinolytic activity from 30 to 60 °C and showed an optimal pH of 8.0. The gelatin-hydrolyzing activity of sMP was inhibited by ethylenediaminetetraacetic acid (EDTA), dithiothreitol (DTT), l-Cys, and l-His. Ca2+ enhanced the proteinase activity, while a higher concentration of Zn2+ inhibited this activity. Peptide mass fingerprinting revealed that 14 fragments were identical to MMP-2 from grass carp (Ctenopharyngodon idella). The full length of the cDNA sequence encoding sMP was 3005 bps, with an open reading frame (ORF) of 1977 bps encoding 658 amino acid residues. Further, sMP could effectively hydrolyze type I collagen even at 4 °C; its activity remained steady during the entire storage period at 4 °C. These results indicated that sMP is MMP-2 and may have been involved in the protein degradation of fish muscle during the postmortem stage. © 2016 Elsevier Ltd.

Keyword:

Characterization; Collagen; Matrix metalloproteinase; Postmortem tenderization; Silver carp

Community:

  • [ 1 ] [Wu, J.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
  • [ 2 ] [Wang, S.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
  • [ 3 ] [Sun, X.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
  • [ 4 ] [Cao, M.]College of Food and Biological Engineering, Key Laboratory of Science and Technology for Aquaculture and Food Safety, Jimei University, Xiamen, 361021, China
  • [ 5 ] [Chen, M.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
  • [ 6 ] [Wang, J.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
  • [ 7 ] [Zhang, Q.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China

Reprint 's Address:

  • [Wu, J.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou UniversityChina

Show more details

Related Keywords:

Related Article:

Source :

Process Biochemistry

ISSN: 1359-5113

Year: 2016

Issue: 7

Volume: 51

Page: 854-864

2 . 4 9 7

JCR@2016

3 . 7 0 0

JCR@2023

ESI HC Threshold:253

JCR Journal Grade:2

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 8

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 3

Affiliated Colleges:

Online/Total:185/10019324
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1