Flavonoids　are　important　active　ingredients　in　many　traditional　Chinese　medicines.　In　this　paper,　capillary　electrophoresis　with　amperometric　detection　was　employed　to　separate　and　detect　eight　flavonoids,　rutin,　quercetrin,　quercetin,　kaempferol,　kaempferide,　catechin,　apigenin,　and　luteolin,　in　a　home-made　capillary　electrophoresis　device.　Under　the　separation　voltage　of　2000　V,　the　eight　flavonoids　could　be　completely　separated　within　33　min　in　18　mM　borax　running　buffer　at　pH　10.2.　Good　linear　relationships　were　obtained　for　all　analytes　and　the　detection　limits　for　flavonoids　ranged　from　0.46　to　0.85　μM.　Then,　the　method　was　applied　to　separate　and　determine　the　flavonoids　in　three　traditional　Chinese　medicines,　hippophae　rhamnoides,　hypericum　perforatum,　and　cacumen　platycladi.　Finally,　rutin,　kaempferol,　quercetin,　and　quercetrin　were　discovered　in　these　medicines　and　the　concentrations　ranged　from　0.28　to　9.94　mg/g.　The　recoveries　of　flavonoids　ranged　from　84.7　to　113%,　which　showed　the　high　reliability　of　this　method.　©　2016　WILEY-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim.