Integration of target responsive hydrogel with cascaded enzymatic reactions and microfluidic paper-based analytic devices (μPADs) for point-of-care testing (POCT) - Details

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Abstract：

Paper　based　microfluidics　(μPADs)　with　advantages　of　portability,　low　cost,　and　ease　of　use　have　attracted　extensive　attention.　Here　we　describe　a　novel　method　that　integrates　glucoamylase-trapped　aptamer-crosslinked　hydrogel　for　molecular　recognition　with　cascaded　enzymatic　reactions　for　signal　amplification　and　a　μPAD　for　portable　readout.　Upon　target　introduction,　the　hydrogel　decomposes　to　release　glucoamylase,　which　catalyzes　the　hydrolysis　of　amylose　to　produce　a　large　amount　of　glucose.　With　a　simple　folding　of　the　μPAD,　the　sample　solution　containing　glucose　product　wicks　and　diffuses　in　parallel　to　each　test-zone　to　carry　out　homogeneous　assays,　where　glucose　is　used　to　produce　I2　for　brown　color　visualization　through　multiple　enzymatic　and　chemical　cascade　reactions.　Through　color　gradient　changes　based　on　different　concentrations　of　the　target,　a　semiquantitative　assay　is　achieved　by　the　naked　eye,　and　quantitation　can　be　obtained　by　handheld　devices.　Detection　of　cocaine　in　buffer　and　urine　was　performed　to　demonstrate　the　utility　of　the　hydrogel-μPAD　system.　More　importantly,　the　hydrogel-μPAD　system　can　be　extended　to　the　detection　of　various　targets　by　incorporating　the　corresponding　aptamer　into　the　hydrogel.　The　hydrogel-μPAD　system　reported　here　provides　a　new　platform　for　portable,　disposable　and　visual　detection　of　a　wide　range　of　targets.　©　2015　Elsevier　B.V.

Keyword：

Aptamer; Cascaded enzymatic reactions; Hydrogel; Point-of-care; μPAD

Community：

[ 1 ] [Tian, T.]MOE Key Lab. of Spectrochemical Analysis, Collab. Innovation Ctr. Chemistry for Energy Materials, Key Lab. Chemical Biology of Fujian Province, State Key Lab. of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China
[ 2 ] [Wei, X.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
[ 3 ] [Jia, S.]MOE Key Lab. of Spectrochemical Analysis, Collab. Innovation Ctr. Chemistry for Energy Materials, Key Lab. Chemical Biology of Fujian Province, State Key Lab. of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China
[ 4 ] [Zhang, R.]MOE Key Lab. of Spectrochemical Analysis, Collab. Innovation Ctr. Chemistry for Energy Materials, Key Lab. Chemical Biology of Fujian Province, State Key Lab. of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China
[ 5 ] [Li, J.]MOE Key Lab. of Spectrochemical Analysis, Collab. Innovation Ctr. Chemistry for Energy Materials, Key Lab. Chemical Biology of Fujian Province, State Key Lab. of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China
[ 6 ] [Zhu, Z.]MOE Key Lab. of Spectrochemical Analysis, Collab. Innovation Ctr. Chemistry for Energy Materials, Key Lab. Chemical Biology of Fujian Province, State Key Lab. of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China
[ 7 ] [Zhang, H.]MOE Key Lab. of Spectrochemical Analysis, Collab. Innovation Ctr. Chemistry for Energy Materials, Key Lab. Chemical Biology of Fujian Province, State Key Lab. of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China
[ 8 ] [Ma, Y.]MOE Key Lab. of Spectrochemical Analysis, Collab. Innovation Ctr. Chemistry for Energy Materials, Key Lab. Chemical Biology of Fujian Province, State Key Lab. of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China
[ 9 ] [Lin, Z.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
[ 10 ] [Yang, C.J.]MOE Key Lab. of Spectrochemical Analysis, Collab. Innovation Ctr. Chemistry for Energy Materials, Key Lab. Chemical Biology of Fujian Province, State Key Lab. of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China

Reprint 's Address：

[Zhu, Z.]MOE Key Lab. of Spectrochemical Analysis, Collab. Innovation Ctr. Chemistry for Energy Materials, Key Lab. Chemical Biology of Fujian Province, State Key Lab. of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen UniversityChina

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Biosensors and Bioelectronics

ISSN： 0956-5663

Year： 2016

Volume： 77

Page： 537-542

7 . 7 8

JCR@2016

1 0 . 7 0 0

JCR@2023

ESI HC Threshold：235

JCR Journal Grade：1

CAS Journal Grade：1

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WoS CC Cited Count：

SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 1

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