Biodegradable　fish　skin　gelatin-titanium　dioxide　(TiO2)　nanocomposite　films　were　fabricated　and　characterized　as　a　function　of　incorporating　amount　of　TiO2　nanoparticles　(gelatin/TiO2　ratio　of　30:1,　20:1　and　10:1).　A　uniform　distribution　of　TiO2　nanoparticles　into　gelatin　matrix　was　observed　using　atomic　force　microscopy　(AFM)　micrographs.　The　data　of　intrinsic　fluorescence　spectra,　Fourier　transform　infrared　spectra　(FTIR)　and　X-ray　diffraction　confirmed　the　interaction　between　protein　and　nanoparticles　through　hydrogen　bonding.　The　TiO2-incorporated　gelatin　nanocomposite　films　exhibited　more　effective　antibacterial　activity　for　Escherichia　coli　after　irradiating　120min　by　UV　light　(365nm),　which　were　54.38%　for　E.　coli　and　44.89%　for　Staphylococcus　aureus,　respectively.　The　analysis　of　physical　properties　revealed　that　addition　of　TiO2　nanoparticles　to　gelatin　films　significantly　increased　the　tensile　strength　and　elongation　at　break,　while　decreased　its　water　vapor　permeability.　The　light　barrier　measurements　indicated　that　these　films　were　highly　transparent,　and　they　had　excellent　barrier　properties　against　UVC　light　at　the　same　time.　The　results　demonstrated　the　feasibility　of　incorporating　nanoparticles　to　improve　the　properties　of　gelatin　films,　and　it　is　of　significance　in　utilizing　the　gelatin　and　titanium　dioxide　to　produce　biodegradable　nanocomposite　film　as　packaging　material　in　food　industry.　©　2015　Elsevier　B.V.