The　efficient　extraction　of　targets　from　complex　surfaces　is　vital　for　technological　applications　ranging　from　environmental　pollutant　monitoring　to　analysis　of　explosive　traces　and　pesticide　residues.　In　our　present　study,　we　proposed　a　proof-of-concept　surface　enhance　Raman　scattering　(SERS)　active　substrate　serving　directly　to　the　rapid　extraction　and　detection　of　target　molecules.　The　novel　substrate　was　constructed　by　decorating　the　commercial　tape　with　colloidal　gold　nanoparticles　(Au　NPs),　which　simultaneously　provides　SERS　activity　and　＂sticky＂　of　adhesive.　The　utility　of　SERS　tape　was　demonstrated　by　directly　extracting　pesticide　residues　in　fruits　and　vegetables　via　a　simple　and　viable　＂paste　and　peel　off＂　approach.　The　obtained　strong　and　easily　distinguishable　SERS　signals　allow　us　to　detect　various　pesticide　residues　such　as　parathion-methyl,　thiram,　and　chlorpyrifos　in　the　real　samples　with　complex　surfaces　including　green　vegetable,　cucumber,　orange,　and　apple.　©　2016　American　Chemical　Society.