A　multi-layer　scaffold　that　incorporates　bioglass,　phosphatidylserine　and　steroidal　saponins　loaded　collagen　microparticles　was　prepared　in　this　study.　Combinatorial　processing　techniques　involving　porogen　leaching　protocol　and　freeze-drying　were　used.　Drug　distribution　and　microstructure　could　be　controlled　mainly　by　the　concentrations　of　drug-loaded　collagen　microparticles　and　porogen　as　well　as　freezing　temperature　for　different　layers　of　the　scaffolds.　Morphological　observation,　measurement　of　swelling　properties,　examination　of　drug　release　kinetics,　and　analysis　of　cell　bioactivity　showed　that　the　resultant　scaffolds　had　highly　interconnected　pores,　gradient　drug　distribution　and　graded　porous　micro-Architecture,　similar　swelling　ratio　for　different　layers,　better　drug　release　kinetics　in　view　of　lower　initial　release　and　slower　average　release　rate　compared　to　the　scaffolds　with　homogeneous　structure,　as　well　as　approving　support　of　cell　ingrowth.　This　study　suggests　that　the　optimized　scaffolds　have　promising　potential　for　applications　in　bone　tissue　engineering.