Peptide-calcium　can　probably　be　a　suitable　supplement　to　improve　calcium　absorption　in　the　human　body.　In　this　study,　a　specific　peptide　Phe-Tyr　(FY)　with　calcium-binding　capacity　was　purified　from　Schizochytrium　sp.　protein　hydrolysates　through　gel　filtration　chromatography　and　reversed　phase　HPLC.　The　calcium-binding　capacity　of　FY　reached　128.77　±　2.57　μg/mg.　Results　of　ultraviolet　spectroscopy,　fluorescence　spectroscopy,　and　infrared　spectroscopy　showed　that　carboxyl　groups,　amino　groups,　and　amido　groups　were　the　major　chelating　sites.　FY-Ca　exhibited　excellent　thermal　stability　and　solubility,　which　were　beneficial　to　be　absorbed　and　transported　in　the　basic　intestinal　tract　of　the　human　body.　Moreover,　the　calcium　bioavailability　in　Caco-2　cells　showed　that　FY-Ca　could　enhance　calcium　uptake　efficiency　by　more　than　three　times　when　compared　with　CaCl2,　and　protect　calcium　ions　against　dietary　inhibitors,　such　as　tannic　acid,　oxalate,　phytate,　and　Zn2+.　Our　findings　further　the　progress　of　algae-based　peptide-calcium,　suggesting　that　FY-Ca　has　the　potential　to　be　developed　as　functionally　nutraceutical　additives.　©　2017　by　the　authors.