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author:

Yang, L. (Yang, L..) [1] | Zhang, Y. (Zhang, Y..) [2] | Li, R. (Li, R..) [3] | Lin, C. (Lin, C..) [4] | Guo, L. (Guo, L..) [5] | Qiu, B. (Qiu, B..) [6] | Lin, Z. (Lin, Z..) [7] | Chen, G. (Chen, G..) [8]

Indexed by:

Scopus

Abstract:

Determination of ochratoxin A (OTA) is highly important for food safety control. In this study, a signal-on electrochemiluminescence (ECL) biosensor which combined the characteristics of high efficiency of hyperbranched rolling circle amplification (HRCA) and high selectivity of aptamer was developed for OTA determination. The capture probe DNA (CDNA) was firstly immobilized on the gold electrode surface through Au-S interaction, then the OTA aptamer was modified on the electrode surface through hybridization with CDNA. Since OTA can competitively bind with the aptamer due to their high affinity, which would induce the releasing of aptamer from the electrode surface. Subsequently, the free CDNA on the electrode surface can hybridize with the padlock probe and induce HRCA reaction subsequently. Thus, the HRCA products which contained large amount of double-stranded DNA (dsDNA) fragments can be accumulated on the electrode surface. Since Ru(phen)32+ can intercalate into the groove of dsDNA and acts as ECL indicator, high ECL intensity can be detected from the electrode surface. The enhanced ECL intensity has a linear relationship with OTA in the range of 0.05-500. pg/mL with a correlation coefficient of 0.9957, and the limit of detection (LOD) was 0.02. pg/mL. The developed biosensor has been applied to determine OTA concentration in the corn samples with satisfied results. © 2015 Elsevier B.V.

Keyword:

Aptamer; Corn; Electrochemiluminescence; Hyperbranched rolling circle amplification; Ochratoxin A

Community:

  • [ 1 ] [Yang, L.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
  • [ 2 ] [Zhang, Y.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
  • [ 3 ] [Li, R.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
  • [ 4 ] [Lin, C.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
  • [ 5 ] [Guo, L.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
  • [ 6 ] [Qiu, B.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
  • [ 7 ] [Lin, Z.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China
  • [ 8 ] [Chen, G.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China

Reprint 's Address:

  • [Lin, Z.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou UniversityChina

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Source :

Biosensors and Bioelectronics

ISSN: 0956-5663

Year: 2015

Volume: 70

Page: 268-274

7 . 4 7 6

JCR@2015

1 0 . 7 0 0

JCR@2023

ESI HC Threshold:265

JCR Journal Grade:1

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 105

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 3

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