Antitumor effects and related mechanisms of Penicitrinine A, a novel alkaloid with a unique spiro skeleton from the marine fungus Penicillium citrinum - Details

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Abstract：

Penicitrinine　A,　a　novel　alkaloid　with　a　unique　spiro　skeleton,　was　isolated　from　a　marine-derived　fungus　Penicillium　citrinum.　In　this　study,　the　isolation,　structure　and　biosynthetic　pathway　elucidation　of　the　new　compound　were　described.　This　new　compound　showed　anti-proliferative　activity　on　multiple　tumor　types.　Among　them,　the　human　malignant　melanoma　cell　A-375　was　confirmed　to　be　the　most　sensitive.　Morphologic　evaluation,　apoptosis　rate　analysis,　Western　blot　and　real-time　quantitative　PCR　(RT-qPCR)　results　showed　penicitrinine　A　could　significantly　induce　A-375　cell　apoptosis　by　decreasing　the　expression　of　Bcl-2　and　increasing　the　expression　of　Bax.　Moreover,　we　investigated　the　anti-metastatic　effects　of　penicitrinine　A　in　A-375　cells　by　wound　healing　assay,　trans-well　assay,　Western　blot　and　RT-qPCR.　The　results　showed　penicitrinine　A　significantly　suppressed　metastatic　activity　of　A-375　cells　by　regulating　the　expression　of　MMP-9　and　its　specific　inhibitor　TIMP-1.　These　findings　suggested　that　penicitrinine　A　might　serve　as　a　potential　antitumor　agent,　which　could　inhibit　the　proliferation　and　metastasis　of　tumor　cells.　©　2015　by　the　authors;　licensee　MDPI,　Basel,　Switzerland.

Keyword：

Anti-metastatic; Anticancer activity; Apoptosis; Human malignant melanoma cell A-375; Marine-derived fungus; Penicitrinine A

Community：

[ 1 ] [Liu, Q.-Y.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
[ 2 ] [Liu, Q.-Y.]Fujian Provincial Key Laboratory of Tumor Biotherapy, Fujian Provincial Tumor Hospital, Fuzhou, 350014, China
[ 3 ] [Zhou, T.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
[ 4 ] [Zhao, Y.-Y.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
[ 5 ] [Chen, L.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
[ 6 ] [Gong, M.-W.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
[ 7 ] [Xia, Q.-W.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
[ 8 ] [Ying, M.-G.]Fujian Provincial Key Laboratory of Tumor Biotherapy, Fujian Provincial Tumor Hospital, Fuzhou, 350014, China
[ 9 ] [Zheng, Q.-H.]Fujian Provincial Key Laboratory of Tumor Biotherapy, Fujian Provincial Tumor Hospital, Fuzhou, 350014, China
[ 10 ] [Zhang, Q.-Q.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, 350002, China
[ 11 ] [Zhang, Q.-Q.]Institute of Biomedical Engineering, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin, 300192, China

Reprint 's Address：

[Chen, L.]Institute of Biomedical and Pharmaceutical Technology, Fuzhou UniversityChina

Email：

ibptcl@fzu.edu.cn

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Source ：

Marine Drugs

ISSN： 1660-3397

Year： 2015

Issue： 8

Volume： 13

Page： 4733-4753

3 . 3 4 5

JCR@2015

4 . 9 0 0

JCR@2023

ESI HC Threshold：193

JCR Journal Grade：1

CAS Journal Grade：2

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SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 4

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