Fruit　development　is　influenced　greatly　by　endogenous　hormones　including　salicylic　acid　(SA)　and　abscisic　acid　(ABA).　Mass　spectrometry　with　high　sensitivity　has　become　a　routine　technology　to　analyze　hormones.　However,　pretreatment　of　plant　samples　remains　a　difficult　problem.　Thus,　dispersive　liquid-liquid　microextraction　(DLLME)　was　used　to　concentrate　trace　plant　hormones　before　liquid　chromatography-ion　trap　mass　spectrometry　(LC-ITMS)　analysis.　Standard　curves　were　linear　within　the　ranges　of　0.5-50,　0.2-20.　ng/mL　for　SA　and　ABA,　respectively.　The　correlation　coefficients　were　greater　than　0.9995　with　recoveries　above　87.5%.　The　limits　of　detection　were　0.2.　ng/mL　for　SA　and　0.1.　ng/mL　for　ABA　in　spiked　water　solution,　respectively　(injection　20.　μL).　The　successful　analysis　of　SA　and　ABA　in　fruit　samples　indicated　our　DLLME-LC-ITMS　approach　was　efficient,　allowing　reliable　quantification　of　both　two　compounds　from　very　small　amounts　of　plant　material.　Moreover,　this　research　revealed　the　relationship　between　SA　and　ABA　content　and　development　of　peach　fruit　at　different　growth　stages.　©　2015.