A one-step electrochemiluminescence immunosensor preparation for ultrasensitive detection of carbohydrate antigen 19-9 based on multi-functionalized graphene oxide - Details

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Abstract：

A　one-step　electrochemiluminescence　(ECL)　immunosensor　for　ultrasensitive　detection　of　carbohydrate　antigen　19-9　(CA19-9)　was　developed　based　on　multi-functionalized　graphene　oxide　(GO),　which　was　prepared　with　N-(4-aminobutyl)-N-ethylisoluminol　(ABEI)　and　CA19-9　antibody　(anti-CA19-9)　chemically　bound　to　the　surface　of　magnetic　GO　(nanoFe3O4　at　GO).　ABEI　and　anti-CA19-9　acted　as　the　electrochemiluminophore　and　the　capture　device　for　CA19-9　respectively.　NanoFe3O4　at　GO　enabled　all　the　ABEI　immobilized　molecules　electrochemically　active　due　to　its　good　conductivity,　and　brought　multi-functionalized　GO　attracted　on　the　surface　of　magnetic　glass　carbon　electrode　through　magnetism.　Thus　the　ECL　immunosensor　could　be　prepared　through　a　one-step　process　that　facilitates　ultrasensitive　detection　of　CA19-9.　Under　optimal　conditions,　the　ECL　intensity　of　the　immunosensor　decreased　proportionally　to　the　logarithmic　concentrations　of　CA19-9　in　the　range　of　0.001-5U/mL　with　a　detection　limit　of　0.0005U/mL.　This　one-step　ECL　immunosensor　showed　good　performance　in　specificity,　stability,　reproducibility,　regeneration　and　application.　It　opened　a　new　avenue　to　apply　multi-functionalized　bionanomaterials　in　ECL　immunoassay.　©　2014　Elsevier　B.V.

Keyword：

Carbohydrate antigen 19-9; Electrochemiluminescence immunosensor; Multi-functionalized graphene oxide; N-(4-aminobutyl)-N-ethylisoluminol; One-step preparation

Community：

[ 1 ] [Sha, Y.]Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo, 315211, China
[ 2 ] [Guo, Z.]Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo, 315211, China
[ 3 ] [Chen, B.]Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo, 315211, China
[ 4 ] [Wang, S.]Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo, 315211, China
[ 5 ] [Ge, G.]Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo, 315211, China
[ 6 ] [Qiu, B.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350002, China
[ 7 ] [Jiang, X.]School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen, 518055, China

Reprint 's Address：

[Guo, Z.]Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo UniversityChina

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Biosensors and Bioelectronics

ISSN： 0956-5663

Year： 2015

Volume： 66

Page： 468-473

7 . 4 7 6

JCR@2015

1 0 . 7 0 0

JCR@2023

ESI HC Threshold：265

JCR Journal Grade：1

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 50

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 1

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