Graphene　oxide　(GO)　nanosheets　were　incorporated　into　an　organic　polymer　monolith　containing　3-acrylamidophenylboronic　acid　(AAPBA)　and　pentaerythritol　triacrylate　(PETA)　to　form　a　novel　monolithic　stationary　phase　for　CEC.　The　effects　of　the　mass　ratio　of　AAPBA/PETA,　the　amount　of　GO,　and　the　volume　of　porogen　on　the　morphology,　permeability　and　pore　properties　of　the　prepared　poly(AAPBA-GO-PETA)　monoliths　were　investigated.　A　series　of　test　compounds　including　amides,　alkylbenzenes,　polycyclic　aromatics,　phenols,　and　anilines　were　used　to　evaluate　and　compare　the　separation　performances　of　the　poly(AAPBA-GO-PETA)　and　the　parent　poly(AAPBA-co-PETA)　monoliths.　The　results　indicated　that　incorporation　of　GO　into　monolithic　column　exhibited　much　higher　resolutions　(＞1.5)　and　column　efficiency　(62000　∼　110000　plates/m　for　toluene,　DMF,　formamide,　and　thiourea)　than　the　poly(AAPBA-co-PETA).　The　successful　application　in　isocratic　separation　of　peptides　suggests　the　potential　of　the　GO　incorporated　monolithic　column　in　complex　sample　analysis.　In　addition,　the　reproducibility　and　stability　of　the　prepared　poly(AAPBA-GO-PETA)　monolith　was　assessed.　The　run-to-run,　column-to-column　and　batch-to-batch　reproducibilities　of　this　monolith　for　alkylbenzenes＇　retention　were　satisfactory　with　the　RSDs　less　than　1.8%　(n　=　5),　3.7%　and　5.6%　(n　=　3),　respectively,　indicating　the　effectiveness　and　practicability　of　the　proposed　method.　©　2014　WILEY-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim.