The　objective　of　this　study　was　to　investigate　the　therapeutic　potential　of　nano-bioglass/phosphatidylserine/collagen　(nBG/PS/COL)　scaffolds　loaded　with　steroidal　saponins　as　an　inducer　factor　for　skeletal　defects.　The　drugs-encapsulated　bone　substitute　was　prepared　by　loading　steroidal　saponins-collagen　microsphere　suspension　in　nano-bioglass　and　phosphatidylserine　(PS)　composite.　The　scaffolds　possess　an　interconnected　porous　structure　with　a　porosity　of　about　82.3%.　The　pore　size　ranges　from　several　micrometers　up　to　about　400　μm.　The　drug　release　assays　showed　the　long-term　sustained　release　of　steroidal　saponins　from　the　scaffolds　with　effective　and　safe　bioactivity.　Moreover,　in　vitro　and　in　vivo　studies　showed　that　the　involvement　of　steroidal　saponins　contributed　to　the　secretion　of　nerve　growth　factor　(NGF)　in　MC3T3-E1　cells,　which　may　be　the　possible　factor　that　greatly　enhanced　bone　healing.　The　results　suggest　that　the　bone　substitute　is　an　effective　implantable　drug-delivery　system　for　use　in　bone　repair.　©　2017　Walter　de　Gruyter　GmbH,　Berlin/Boston.