We　present　the　synthesis　of　graphene　oxide　(GO)　via　the　modified　Hummer　method　and　nanocomposites　based　on　collagen/nano-hydroxyapatite　(COL/nHA).　GO　was　prepared　by　freeze　drying　the　stable　mixture　of　the　components.　Comprehensive　characterizations　of　the　properties　of　GO　were　conducted　by　X-ray　diffraction　and　Fourier-transform　infrared　spectra　analyzer.　Effects　of　the　incorporation　of　GO　on　morphology,　phase　structure,　and　wear　resistance　of　the　resulting　composite　coatings　were　evaluated　in　detail.　FT-IR　results　show　that　the　GO　and　nHA　disperse　homogeneously　in　the　composite.　COL/GO/nHA　composites　with　high　porosity　were　confirmed　by　scanning　electron　microscope　and　porosity　test.　By　increasing　the　GO　content　from　0　to　4　wt%,　COL/GO/nHA　composites　exhibited　improved　hydrophilic　and　mechanical　properties.　The　cell　viability　and　proliferation　results　showed　that　osteoblastic　cells　can　adhere　and　develop　on　COL/GO/nHA　composites.　An　outstanding　proliferation　potential　was　displayed　by　the　cells　in　contact　with　COL/GO/nHA　4　wt%.　The　GO-reinforced　COL/nHAs　with　high　mechanical　and　bioactive　properties　are　potential　candidates　for　bone　tissue　engineering.　©　2017　American　Scientific　Publishers　All　rights　reserved.