Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots - Details

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Abstract：

Advances　in　the　development　of　small　photoblinking　semiconducting　polymer　dots　(Pdots)　have　attracted　great　interest　for　use　in　super-resolution　microscopy.　However,　multicolor　super-resolution　imaging　using　conventional　small　photoblinking　Pdots　remains　a　challenge　due　to　their　limited　color　choice,　broad　emission　spectrum,　and　heavy　spectrum　crosstalk.　Here,　we　introduce　two　types　of　small　photoblinking　Pdots　with　different　colors　and　relatively　narrow　emission　spectra:　blue　PFO　Pdots　and　carmine　PFTBT5　Pdots　for　blinking-based　statistical　nanoscopy.　Both　of　these　probes　feature　ultrahigh　single-particle　brightness,　very　strong　photostability,　superior　biocompatibility,　and　robust　fluorescence　fluctuation.　In　addition,　these　small　photoblinking　Pdots　serve　as　excellent　labels　for　dual-color　super-resolution　optical　fluctuation　imaging　(SOFI)　of　specific　subcellular　structures,　indicating　their　promise　for　long-term　multicolor　SOFI　nanoscopy　with　high　spatiotemporal　resolution.　©　2017　American　Chemical　Society.

Keyword：

bioimaging; blue and carmine small polymer dots; multicolor super-resolution imaging; photoblinking; super-resolution optical fluctuation imaging

Community：

[ 1 ] [Chen, X.]State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China
[ 2 ] [Liu, Z.]Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 510855, China
[ 3 ] [Liu, Z.]State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China
[ 4 ] [Li, R.]State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China
[ 5 ] [Shan, C.]State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China
[ 6 ] [Zeng, Z.]College of Physics and Information Engineering, Fuzhou University, Fuzhou, 350116, China
[ 7 ] [Xue, B.]State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China
[ 8 ] [Yuan, W.]State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China
[ 9 ] [Mo, C.]State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China
[ 10 ] [Xi, P.]State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China
[ 11 ] [Wu, C.]Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 510855, China
[ 12 ] [Sun, Y.]State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, 100871, China

Reprint 's Address：

[Xi, P.]State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Department of Biomedical Engineering, College of Engineering, Peking UniversityChina

Email：

xipeng@pku.edu.cn

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Source ：

ACS Nano

ISSN： 1936-0851

Year： 2017

Issue： 8

Volume： 11

Page： 8084-8091

1 3 . 7 0 9

JCR@2017

1 5 . 8 0 0

JCR@2023

ESI HC Threshold：306

JCR Journal Grade：1

CAS Journal Grade：1

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 74

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 0

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