The　authors　describe　a　colorimetric　method　for　the　determination　of　cholesterol　in　human　serum.　Cholesterol　is　enzymatically　oxidized　by　oxygen　in　the　presence　of　cholesterol　oxidase　(ChOx)　to　produce　4-cholestene-3-one　and　hydrogen　peroxide　(H2O2).　Due　to　the　peroxidase-like　activity　of　MoS2　nanosheets,　H2O2　can　oxidize　the　chromogenic　substrate　3,3′,5,5′-tetramethylbenzidine　(TMB)　to　give　a　blue　product.　The　increase　in　the　absorbance　of　the　acidic　oxTMB　solution　at　450 nm　is　used　for　quantification　of　cholesterol.　Under　the　optimal　conditions,　the　increase　in　absorbance　is　proportional　to　the　concentration　of　cholesterol　in　the　range　from　2　to　200 μmol⋅L−1,　and　the　limit　of　detection　is　0.76 μmol⋅L−1.　The　color　change　from　pale　yellow　to　blue　color　can　also　be　detected　visually　for　cholesterol　concentrations　as　low　as　20 μmol⋅L−1.　Such　an　enzyme　mimetic-based　assay　possesses　advantages　over　enzyme-based　assays　in　terms　of　costs,　stability　against　denaturation,　and　protease　digestion.　The　assay　was　applied　to　the　determination　of　free　cholesterol　and　of　total　cholesterol　(after　hydrolysis　using　an　esterase)　in　spiked　human　serum,　and　it　gave　satisfactory　recoveries.　[Figure　not　available:　see　fulltext.]　©　2017,　Springer-Verlag　Wien.