The　authors　describe　an　enzyme-free　amperometric　method　for　the　determination　of　glucose　at　nanomolar　levels　at　near　neutral　pH　values.　A　hybrid　nanostructure　composed　of　molybdenum　disulfide　and　copper　sulfide　(MoS2-CuS)　was　prepared　using　L-cysteine　as　both　the　sulfur　donor　and　the　reducing　agent.　The　nanohybrid　was　then　immobilized　on　a　glassy　carbon　electrode　(GCE)　by　incorporating　it　into　a　film　of　poly(vinyl　butyral).　Transmission　electron　microscopy　and　Raman　spectroscopy　were　utilized　to　characterize　the　MoS2-CuS　nanohybrids.　Three　modified　GCEs　(MoS2/GCE,　CuS/GCE　and　MoS2-CuS/GCE)　were　investigated　with　respect　to　their　sensitivity　to　glucose,　and　the　MoS2-CuS/GCE　was　found　to　perform　best　in　displaying　a　limit　of　detection　as　low　as　0.3 μM　in　pH 7.2　buffer　at　an　applied　potential　of　+0.18 V　(versus　Ag/AgCl).　The　repeatability　and　intermediate　precision　are　below　7.0%　at　0.05,　0.5　and　1.0 mM　concentration　levels.　The　method　was　applied　to　the　determination　of　glucose　in　spiked　human　serum　samples,　and　recoveries　were　between　92.3　and　110.7%.　This　detection　scheme　is　rapid　and　cost-effective.　Natural　enzymes　and　additional　electron　mediators　are　not　required.　[Figure　not　available:　see　fulltext.]　©　2017,　Springer-Verlag　Wien.